U.S. Department of Energy

Pacific Northwest National Laboratory

Quantitative Metabolomics Using NMR

Monday, September 15, 2014
Dr. David Chang
The field of metabolomics started by studying small molecule data from nuclear magnetic resonance (NMR) and mass spectrometry (MS) experiments. Often this raw data is studied by the use of chemometrics, or multivariate statistics, as performance of these methods is quick. However, as more and more biological questions are asked of these data, researchers have the need to both identify and quantify the metabolites present in the data before the statistical analysis. Absolute quantitation also allows this type of research to be translatable and reproducible, both between different locations and between different platforms or analytical techniques. There are many challenges associated with accurate quantitative metabolomics. This presentation will address specifically the use of NMR and the requirements for accurate quantitation, as well as address some published work on known variations in these data.
Speaker Bio

David Chang has been active in the area of metabolomics for over ten years. He is currently the Director of Products and Services at Chenomx Inc., a metabolomics company offering both software and services related to this field. David received both his Bachelor of Applied Science degree and Masters of Applied Science degree in Chemical Engineering from the University of Waterloo. In the early 2000s David moved to Edmonton, Alberta to pursue his doctorate at the University of Alberta. It was at the University of Alberta that David was first involved in the early discoveries of using Nuclear Magnetic Resonance for metabolomics. His expertise in signal processing, multivariate statistics, and chemometrics was brought on to help researchers in biochemistry and medicine develop the tools needed to interpret the complex frequency domain signals of NMR spectroscopy. His expertise in modeling and statistics also helped researchers interpret the vast amounts of quantitative metabolomics data, which has led to the beginnings of many biomarker diagnostic applications. David joined Chenomx in 2007 to build a contract services group. David leads both the services group as well as software development group at Chenomx.


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